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seanspotatobusiness

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  1. Hi. I was hoping someone could advise me how to affix a motor to a base plate as shown in the picture. The problem is that with the motor spindle/shaft in position, the blocks holding the motor no longer line up.
  2. We don't have any shaking incubators that also provide a 5% CO2 atmosphere. The only shaking incubators we have are for microbiology (no CO2).The temperature will be 39 C and the relative humidity will be ~70%. I will pre-heat the device in a sealed plastic bag to prevent the formation of condensation on the electrical components. I've bought random Lego Technic off Ebay which I'm hoping will contain the parts I need and will follow up with specific purchases for anything it doesn't. I'll let you all know how it works out if you're interested.
  3. Thanks for the help everyone. Hi. It's difficult to guess what set will fit my needs given that they don't seem to list exactly what parts are in them. It doesn't look like I can easily avoid buying superfluous parts. :(
  4. I don't have any experience using Lego Technic. I work in a life science lab and would like to determine the effect of agitation on transfection efficiency in my cells of interest. I would like to build a fairly simple device which will either rock or swirl a multi-well plate (Figure 1) and be of sufficiently small profile that I can put it inside a cell culture incubator, to run off a battery. The agitation needs to be fairly gentle, as opposed to clunky, to prevent the liquid culture medium from being ejected from the wells. I'll need a way to set the rate of movement (too fast is more likely to be an issue than too slow) but this does not need to be easily adjustable once set. I do not anticipate building anything more complex in the future. Could anyone advise me on the most basic set of components that I can buy that will allow me to achieve this? Many thanks in advice for any guidance. Figure 1. These are example multi-well plates. In use, the wells are 25-35% filled with liquid. They are approximately 86 x 128 mm.
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